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OBJECTIVE@#To evaluate the clinical efficacy of Huangqi Sijunzi decoction (HQSJZD) for treating cancer-related fatigue (CRF) of spleen and stomach Qi deficiency type after chemotherapy in patients with breast cancer.@*METHODS@#A total of 94 breast cancer patients who developed CRF of spleen and stomach Qi deficiency type after chemotherapy were randomized into chemotherapy group (n=47) and traditional Chinese medicine (TCM) + chemotherapy group (n=47). The patients in chemotherapy group received the AC or EC regimen and non-drug interventions including psychological counseling, and those in TCM + chemotherapy group received oral administration of HQSJZD in addition to chemotherapy for 21 days as a treatment cycle, after which improvement of fatigue was assessed using Modified Piper Fatigue Scale. The active ingredients and targets of HQSJZD were screened using the TCM System Pharmacology Analysis Platform (TCMSP); the CRF- and breast cancer-related disease targets were retrieved based on data from the GeneCards, NCBI gene and OMIM databases to construct the component-target network and the protein-protein interaction (PPI) network. GO functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes KEGG pathway enrichment analysis of the target genes were performed to construct the component-disease-pathway-target biological network. The binding strength of the major drug ingredients and CRF key targets were predicted using AutoDock software.@*RESULTS@#The scores for somatic fatigue, emotional fatigue and cognitive fatigue, along with the overall fatigue score, showed more significant improvements in TCM+chemotherapy group than in chemotherapy group (P < 0.001), and the response rate reached 89.4% in the combined treatment group. We identified 250 targets for HQSJZD, 2653 CRF-related genes, 15 329 breast cancer-related genes and 161 prescription-disease intersected targets, from which topological analysis identified 66 potential key targets. GO and KEGG enrichment analyses predicted multiple pathways related with the disease. Molecular docking results suggested that the core ingredients of HQSJZD showed high affinities to the key targets AKT1, CASP3, IL6, JUN and VEGFA, among which AKT1 might be the most important target for HQSJZD to treat CRF.@*CONCLUSION@#HQSJZD can obviously improve CRF symptoms in breast cancer patients possibly by regulating multiple signaling pathways including PI3K-Akt through AKT1.
Subject(s)
Female , Humans , Breast Neoplasms/drug therapy , Drugs, Chinese Herbal/pharmacology , Medicine, Chinese Traditional , Molecular Docking Simulation , Network Pharmacology , Phosphatidylinositol 3-KinasesABSTRACT
This paper summarized the operation cooperation and management of the first case of bilateral lung transplantation assisted with double extracorporeal membrane oxygenation (ECMO) for the recipient with novel coronavirus pneumonia (COVID-19) in the world. The bilateral lung transplantation assisted by double ECMO had been accomplished successfully on a recipient with COVID-19, who was supported by ventilator and ECMO, with other organs function basically normal. The operation took 405 min and went smoothly. The cooperation and management guidelines of the operation room included the following: setting up of COVID-19 emergency operation group quickly; listing and preparing of the detailed operation supplies; transferring of the whole system of lung transplantation operating room in Wuxi People's Hospital to the Wuxi No.5 People's Hospital (isolation hospital); reconstruction of a negative pressure operating room for lung transplantation; formulating and strictly implementing the guidelines and management process for the operation of patient with COVID-19.
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Aim To explore the effect of soybean meal on bone metabolism in ovariectomized osteoporosis rat-sandits mechanism. Methods Three-month-old SPF-class SD female rats were randomly divided into sham operation group, model group, estradiol group and soybean meal administration group, with 8 rats in each group. One week after the operation, the corresponding drug was administered by gavage for 8 weeks. After 8 weeks, the bone mineral density (BMD) and uterus index of each group were measured. Biomarkers for ovariectomized osteoporosis rats were identified by urinary metabolomics. Results (1) Soybean meal could significandy increase BMD and uterine index of femur in ovariectomized rats in model group,and significantly reduce the weight of ovariectomized ratsin model group. (2) 23 biomarkers and 9 metabolic pathways closely related to postmenopausal osteoporosiswere i-dentified. Soybean meal could significantly exert a callback effect on 12 biomarkers by regulating various metabolic pathways, such as steroid hormone biosynthesis metabolism, inositol phosphate metabolism, primary bile acid biosynthesis metabolism and purine metabolism. Conclusions Soybean meal can effectively interfere with the occurrence and development of postmenopausal osteoporosis. This study provides scientific data for the prevention and treatment of postmenopausal osteoporosis drugs.
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Objective@#To explore the effects of comprehensive temperature preserving nursing on the body temperature and the levels of lactic acid in lung transplantation patients.@*Methods@#Totally 46 patients underwent lung transplantation were randomly divided into experimental group and control group, with 23 patients in each group. Comprehensive insulation nursing measures were implemented in the experimental group, and conventional insulation measures were used in the control group. The rectal temperature and arterial blood lactic acid values of the two groups were recorded and compared before anesthesia (T0), after chest opening but before pulmonary artery occlusion (T1), after pulmonary artery occlusion (T2), during new lung transplantation (T3), after pulmonary artery opening (T4) and after surgery (T5).@*Results@#There was no statistically significant difference in rectal temperature and blood lactic acid between the two groups before anesthesia (t=1.53, 0.61, P>0.05). The rectal temperature of the experimental group were (36.55±0.14), (36.42±0.160), (36.33±0.13), (36.15±0.11), (36.45±0.10) ℃, which was higher than that of the control group (35.69±0.17, 35.52±0.19, 34.61±0.17, 34.47±0.13, 34.79±0.18) (F=3.24, P<0.01). The lactic acid level of the experimental group were (1.50±0.10), (2.97±0.15), (3.29±0.15), (2.15±0.10), (1.87±0.13) mmol/L, which was lower than that of the control group (2.37±0.10, 4.07±0.16, 5.15±0.21, 4.85±0.15, 4.72±0.17) (F=1.14, P<0.01). There was no statistically significant difference in rectal temperature between T0 and all operative stages in experimental group (F=0.39-1.41, P>0.05). The lactate levels of experimental group at T2 and T3 was higher than that before anesthesia (F=1.33, 1.16, P<0.01), and recovered to the pre-anesthesia level at T4 and T5 (F=0.59, 1.00, P>0.05).In the control group, the rectal temperature during each operation period was lower than that before anesthesia (F=1.17-2.51, P<0.01), and the lactic acid level was higher than that before anesthesia (F=0.51-2.25, P<0.01).@*Conclusion@#Comprehensive heat preservation nursing measures can maintain the relative stability of body temperature during lung transplantation, which is conducive to tissue perfusion and oxygenation.
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Objective To explore the effects of comprehensive temperature preserving nursing on the body temperature and the levels of lactic acid in lung transplantation patients. Methods Totally 46 patients underwent lung transplantation were randomly divided into experimental group and control group, with 23 patients in each group. Comprehensive insulation nursing measures were implemented in the experimental group, and conventional insulation measures were used in the control group. The rectal temperature and arterial blood lactic acid values of the two groups were recorded and compared before anesthesia (T0), after chest opening but before pulmonary artery occlusion (T1), after pulmonary artery occlusion (T2), during new lung transplantation (T3), after pulmonary artery opening (T4) and after surgery (T5). Results There was no statistically significant difference in rectal temperature and blood lactic acid between the two groups before anesthesia (t=1.53, 0.61, P>0.05). The rectal temperature of the experimental group were (36.55±0.14), (36.42±0.160), (36.33±0.13), (36.15±0.11), (36.45±0.10)℃, which was higher than that of the control group (35.69±0.17, 35.52±0.19, 34.61±0.17, 34.47±0.13, 34.79±0.18) (F=3.24, P<0.01). The lactic acid level of the experimental group were (1.50±0.10), (2.97±0.15), (3.29± 0.15), (2.15±0.10), (1.87±0.13) mmol/L, which was lower than that of the control group (2.37±0.10, 4.07± 0.16, 5.15±0.21, 4.85±0.15, 4.72±0.17) (F=1.14, P<0.01). There was no statistically significant difference in rectal temperature between T0 and all operative stages in experimental group (F=0.39-1.41, P>0.05). The lactate levels of experimental group at T2 and T3 was higher than that before anesthesia (F=1.33, 1.16, P<0.01), and recovered to the pre-anesthesia level at T4 and T5 (F=0.59, 1.00, P>0.05).In the control group, the rectal temperature during each operation period was lower than that before anesthesia (F=1.17-2.51, P<0.01), and the lactic acid level was higher than that before anesthesia (F=0.51-2.25, P<0.01). Conclusion Comprehensive heat preservation nursing measures can maintain the relative stability of body temperature during lung transplantation, which is conducive to tissue perfusion and oxygenation.
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The objective of this work is to synthesize indolacin-5-fluorouracil-1-ylmethyl ester and the structure was confirmed by means of UV, IR, [1]H-NMR, [13]C-NMR and mass spectrometry. The physicochemical parameters of melting point, solubility, apparent partition coefficient were investigated. S180 sarcoma, H22 hapatitic cancer and Lewistransplanted mice were used to evaluate the anti-tumor activity of indolacini-5-fluorouracil-1-ylmethyl ester compared with 5-fluorouracil in vivo. Anti-inflammatory and analgesic activities were evaluated in mice. The inhibitory ratio of indolacini- 5-fluorouracil-1-ylmethyl ester is comparative to that of 5-fluorouracil. This study indicates that 5- fluorouracil-1-ylmethyl ester may represent a new anticancer predrug of 5-fluorouracil to produce a combined effect of indolacin and 5-fluorouracil for cancer therapy
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AIM:To be one of the primary cause injury to multiple sites of ocular of glaucoma which affects over 70 million people worldwide. We applied data mining techniques, linear and the matrix operations, efficiently calculated the network and estimated the possible function of the“node” genes of the retina and optic of glaucoma, in order to provide new thought and method on the pathogenesis of glaucoma. METHODS: The data in this study is from Gene Expression Omnibus ( GEO ) which belong to Nation Center for Biotechnology Information ( NCBI) , the quality of the raw data CEL files was processed and analyzed by the Expression software which belong to Affymetrix Inc. , Santa Clare, CA, USA. Significant analysis method ( SAM) which base on the T test was used to identified the significant genes. Based on GRNInfer and Gvedit soft we set up gene networks of optic and retina of mice and further more enriched analysis which based on DAVID and MAS3. 0 online software were processed. RESULTS:The analysis between the group of the optic nerve heads and retinas in different stage of glaucoma showed that the amount of significant different expressed genes in the optic never head group increased significantly comparing with the group of retina in the early stage of glaucoma, the analysis of the genes network construction show that:the node genes of optic nerve heads included Unc13c、Kif5a、TRPM1、PANX; and the node genes of retina include POU4F1, NEFL, BC03870, CALB2. Metabolic pathways enrichment analysis which based on MAS3. 0 online platform show that there was mainly the amyotrophic lateral sclerosis, tyrosine metabolism, melanogenesis, Nitrogen metabolism, Gap junction, Leukocyte transendothelial migration metabolism pathway enriched out in optic nerve head; and there was mainly amyotrophic lateral sclerosis, neurodegenerative disorders, prostate cancer, leukocyte transendothelial migration metabolism pathway enriched out in retina. CONCLUSION:By understanding bioinformatics result, it seems optic were more sensitive than the retina to high intraocular pressure, and weather high expression of TYrp1 gene can be as a sensitive diagnostic item require more evidence back up. Functional enrich analysis of node gene showed that cytoskeleton reconstructed, molecular motor and nutrients transport function improve in optic; and in retina, the most prominent finding in retina was enrichment function modules were focus on regeneration, repairing and differentiation of cells, which remind that we should reinforce research on reparation of retina of primary glaucoma. Metabolic pathways enrichment analysis show that inflammatory response plays prominent place in optic and retina of primary glaucoma, because of the optic narrow and crowed anatomic shape, nutrient metabolism and substances transfer enrichment modules play an important role in optics of primary glaucoma.
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<p><b>OBJECTIVE</b>To analyze the immunization status of category II vaccine in Chinese Mainland in 2012.</p><p><b>METHODS</b>The completeness of report unit by township and county, including 31 provinces (cities, municipalities) and Xinjiang Production and Construction Corps, the number of doses and the number of counties covered for category II vaccine at different areas, average types of category II vaccine by county were analyzed by descriptive epidemiological methods, according to monthly report of vaccination data for category II vaccines by township in 2012 which all of provinces and population were almost covered, through the National Immunization Program(NIP) monitoring information system of China.</p><p><b>RESULTS</b>A total of 29 kinds of category II vaccine with 90 843 530 doses were reported in 2012, and the total average dose was 674.2 per 10 000 people. The report completeness by county and township were 83.32% (29 557/35 472) and 80.01% (396 652/495 756) respectively. The reported doses of rabies vaccine for human use, Haemophilus influenza type b vaccine and influenza vaccine was the top third vaccine, among those for all kinds of category II vaccine, which were 17 027 259, 13 996 206, 11 324 518 respectively, and 126.4, 103.9, 84.1 doses per 10 000 people. In 2773 county units, varicella attenuated live vaccine, influenza vaccine, rabies vaccine for human use were the top three kinds of category II vaccine in terms of the number of county where vaccines have been used in 2012, which were 2442(88.06%), 2415(87.09%), 2366(85.32%) respectively. Guangdong province with 12 266 531 doses was the highest report doses for category II vaccine whereas Qinghai province with 57 767 doses was the lowest number in 2012. Regarding to the average report doses by province, the highest or lowest number was 2425.0 doses per 10 000 people in Shanghai province, and 101.7 doses per 10 000 people in Qinghai province separately.</p><p><b>CONCLUSION</b>Many kinds of category II vaccine with a large amount have been used in China, and there are significant different among areas. Surveillance and management for category II vaccines should be future improved.</p>
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Humans , China , Epidemiology , Immunization Programs , Population Surveillance , VaccinationABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of the aluminum hydroxide (Al-OH) adjuvant on the 2009 pandemic influenza A/H1N1 (pH1N1) vaccine.</p><p><b>METHODS</b>In a multicenter, double-blind, randomized, placebo-controlled trial, participants received two doses of split-virion formulation containing 15 μg hemagglutinin antigen, with or without aluminum hydroxide (Al-OH). We classified the participants into six age categories (>61 years, 41-60 years, 19-40 years, 13-18 years, 8-12 years, and 3-7 years) and obtained four blood samples from each participant on days 0, 21, 35, and 42 following the first dose of immunization. We assessed vaccine immunogenicity by measuring the geometric mean titer (GMT) of hemagglutination inhibiting antibody. We used a two-level model to evaluate the fixed effect of aluminum Al-OH and other factors, accounting for repeated measures.</p><p><b>RESULTS</b>The predictions of repeated measurement on GMTs of formulations with or without Al-OH, were 80.35 and 112.72, respectively. Al-OH significantly reduced immunogenicity after controlling for time post immunization, age-group and gender.</p><p><b>CONCLUSION</b>The Al-OH adjuvant does not increase but actually reduces the immunogenicity of the split-virion pH1N1 vaccine.</p>
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Adjuvants, Pharmaceutic , Chemistry , Aluminum Hydroxide , Chemistry , Antibodies, Viral , Blood , China , Data Interpretation, Statistical , Double-Blind Method , Hemagglutination Inhibition Tests , Influenza A Virus, H1N1 Subtype , Allergy and Immunology , Influenza Vaccines , Chemistry , Allergy and Immunology , Influenza, Human , Epidemiology , Allergy and Immunology , Virology , Models, Statistical , PandemicsABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Amyloid deposition is rare. If there was a great amount of amyloid depositions in the skin tissue, it would be considered to be amyloid deposition disease at first, and then primary cutaneous marginal zone B-cell lymphoma (PCMZL). This study was to analyze the diagnosis and differential diagnosis of two cases of PCMZL with amyloid deposition.</p><p><b>METHODS</b>Clinicopathologic characteristics and follow-up of two cases of PCMZL were analyzed. Immunohistochemical staining was performed by EnVision method using antibodies LCA, CD19, CD20, CD79a, CD3, CD7, MUM1, kappa, lambda, Ki-67. IgH and TCRgamma gene rearrangement was detected by polymerase chain reactive (PCR).</p><p><b>RESULTS</b>Case 1, a 71-year-old Chinese male, had a subcutaneous mass on the right elbow that was initially diagnosed with "amyloidosis" in 2004. Three years after the initial diagnosis, he developed recurrences on the right para-auxillary that was still diagnosed with "probably amyloidosis". Four years after the first diagnosis, the patient presented a lesion on the right para-auxillary with a diameter of 2 cm and a lesion on the temporal-parietal dural with a size of 6.0 cmx3.0 cmx3.0 cm. Case 2, a 68-year-old Chinese male, had a subcutaneous mass next to back of the left ear with a size of 9.0 cmx5.0 cm, and he underwent a operation one year previously because of subcutaneous mass in the same site. Microscopically, the tumors of both cases were located in dermis and subcutaneous, tumor cells were medium size with a nodular or diffuse distribution, and some of tumor cells were plasmacytoid/plasma cells. Morphologically, the temporal-parietal dural lesion was similar to subcutaneous lesion and infiltrated into cranial (case 1). Juxtaposed the tumor cells of two cases, there were the large amyloid deposits of amorphous hyaline material and concentrically laminated hyaline spherules in case 1, while cord-like amyloid deposits in case 2. Reactive lymphoid follicles with germinal centers and foreign body giant cells in the stroma were found surrounding the amyloid deposits. Congo red staining showed positive of amyloid deposition in tumor tissues of both cases. Immunohistochemical staining revealed that LCA, CD19, CD20, CD79a and MUM1 expressions were positive in tumor cells, and Ki-67 expression was about 8%-10%. IgL restricted expression as kappa positive while lambda negative was found in both cases. PCR results showed monoclone gene rearrangement of IgH gene in both cases.</p><p><b>CONCLUSIONS</b>Our findings suggest that amyloid deposition rarely present in both primary and metastatic tumors in PCMZL, and its diagnosis should be considered to avoid misdiagnosis. The patients with PCMZL should undergo regular examinations and chemotherapy as well as a long-term follow-up since it is apt to recur or relapse.</p>
Subject(s)
Aged , Humans , Male , Amyloidosis , Drug Therapy , Metabolism , Pathology , General Surgery , Antigens, CD , Metabolism , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Cyclophosphamide , Therapeutic Uses , Diagnosis, Differential , Doxorubicin , Therapeutic Uses , Elbow , Follow-Up Studies , Head and Neck Neoplasms , Drug Therapy , Metabolism , Pathology , General Surgery , Interferon Regulatory Factors , Metabolism , Leukocyte Common Antigens , Metabolism , Lymphoma, B-Cell, Marginal Zone , Drug Therapy , Metabolism , Pathology , General Surgery , Magnetic Resonance Imaging , Neoplasm Recurrence, Local , Prednisone , Therapeutic Uses , Skin Neoplasms , Drug Therapy , Metabolism , Pathology , General Surgery , Vincristine , Therapeutic UsesABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunity level of diphtheria antibody among children living in the areas where different coverage rates of 4-vaccines stratified by results of national immunization program (NIP) reviewed in 2004.</p><p><b>METHODS</b>According to data from 4-vaccine coverage rates of NIP reviewed in 2004, 3 levels could be set. We randomly selected 2 counties at each level and then 10 villages from each county with 42 children involved who were born between 1992 and 2003. ELISA quantitative method was used to test IgG of diphtheria antitoxin.</p><p><b>RESULTS</b>(1) The positive rate of diphtheria antitoxin was only 49.6% with the highest as 78.1% and lowest as 33.0%. There was a significant decreasing trend of this positive rate with the increase of age. The highest (61.2%) fell in the group that were born in 2003 and the lowest (37.6%) was seen among children born in 1992 to 1995. (2) Geometric mean concentrations (GMCs) was only 0.48 IU/ml with a trend of decrease when age was increasing. There was no GMCs peak seen in children who were at the age of boosting, as expected. (3) Positive rates of children born between 2001 and 2003 were lower than 62% while the diphtheria-pertussis-tetanus (DPT) vaccine coverage rates were all higher than 90%. (4) There was no significant difference of diphtheria antitoxin positive rates between children with eligible routine immunization (58.1%) and those were ineligible (59.6%).</p><p><b>CONCLUSION</b>Other than some specific ones, children from most of the investigated counties had a low level of antibody against diphtheria. The coverage rate of DPT vaccine did not necessarily reflect the immunity against diphtheria, suggesting the increase of immunity against diphtheria an urgent task to be taken care of.</p>
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Adolescent , Child , Child, Preschool , Female , Humans , Male , Age Distribution , Antibodies, Bacterial , Allergy and Immunology , China , Diphtheria , Allergy and Immunology , Diphtheria Antitoxin , Allergy and Immunology , Diphtheria-Tetanus-Pertussis Vaccine , Allergy and ImmunologyABSTRACT
This study was aimed to investigate the reversible effect of cyclosporine A, raloxifene and their combination on multidrug resistance cell line K562/A02. The IC(50) (the concentration causing 50% inhibition of cell growth) of DNR were assayed by MTT method, the expression level of mdr-1 mRNA was assayed by RT-PCR, p-glycoprotein (P-gp) expression and intracellular DNR concentration were detected by flow cytometry. The results showed that the IC(50) of DNR on K562/A02 and K562 cells were 23.51 mg/L and 0.29 mg/L, respectively. The IC(50) of DNR on K562/A02 cells in treatment with raloxifene CsA and both combination were 5.98, 8.15 and 3.68 mg/L respectively, but both drugs not influenced IC(50) of DNR on K562 cells. Pretreating K562/A02 cells with raloxifene (2.5 mg/L) or CsA (1 mg/L) for 48 hours partially restored the sensitivity of K562/A02 cells to DNR. Cyclosporine A and raloxifene (alone or combination) elevated the intracellular DNR concentration in K562/A02, down regulated P-gp and mdr-1 mRNA expressions. It is concluded that multidrug resistance (MDR) can be partially reversed by CsA or raloxifene, the combination of both drugs shows a great synergistic reversal effect.
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Genetics , Cyclosporine , Pharmacology , Doxorubicin , Pharmacology , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Drug Synergism , K562 Cells , Pathology , RNA, Messenger , Genetics , Raloxifene Hydrochloride , PharmacologyABSTRACT
<p><b>AIM</b>To study the mechanism of the therapeutic angiogenesis effect of bone marrow stromal cells (BMSCs) implantation on rat acute myocardial infarction models.</p><p><b>METHODS</b>The rat acute myocardial infarction models were made by coronary artery ligation and divided into 2 groups at random. In the experiment group, twice passaged BMSCs were labeled with BrdU and then implanted into the infarction region of the recipients in 4 weeks. The control group was the model rats received only DMEM injection. In control group, the hearts were harvested on the day 3, 7, 14, 28, 42 and 56. The infarction regions were examined to identify the angiogenesis and the expression of the VEGF and bFGF. In experiment group, the hearts were examined on the day 42 and 56 after AMI (the day 14 and 28 after cells implantation).</p><p><b>RESULTS</b>Viable cells labeled with BrdU could be identified in host hearts. Histologic examination found most donor cells within the infarction region expressed fibroblastic and endothelial phenotype. The transplantation regions had a greater capillary density than the control regions did (14 +/- 4.7/HPF vs 6 +/- 2.4/HPF, P < 0.05). In the control group, the expression of VEGF and bFGF within the infarction regions peaked on day 7, and then decreased over time. In the experiment groups, the expression of bFGF and VEGF on the day 42 and 56 had a higher level than the control group did.</p><p><b>CONCLUSION</b>The expression of VEGF and bFGF is significantly increased after cells therapy during the late phase of AMI. It indicates that BMSCs implantation promoted the angiogenesis is mediated by its differentiation into endothelium and the increased release of VEGF and bFGF.</p>